MMP-16与骨巨细胞瘤临床相关性的初步研究

目的:研究复发与未复发骨细胞瘤细胞中MMP-16的变化,初步探索MMP-16与骨巨细胞瘤复发之间的联系,进而讨论MMP-16对于骨巨细胞瘤的临床意义。方法:对临床中复发和未复发共50例骨巨细胞瘤患者切除的骨巨细胞瘤组织进行标本采集。首先应用免疫组化荧光染色直观观察复发组和未复发组骨巨细胞瘤组织中MMP-16的表达情况,再运用real-time PCR技术检测MMP-16 mRNA的表达情况,最后应用western blot技术测定MMP-16的含量。从形态、基因表达和蛋白三个方面全面比较复发组和未复发组骨巨细胞瘤组织中MMP-16的表达情况。结果:免疫组化结果显示复发组中MMP-16的表达要明显高于未复发组;real-timePCR结果显示复发组中的MMP-16 mRNA的表达量要明显高于未复发组;western blot结果显示复发组中MMP-16蛋白的表达量要高于未复发组(P0.05)。结论:MMP-16与骨巨细胞瘤存在密切联系,MMP-16的表达增强是骨巨细胞瘤的复发的重要标志,通过对MMP-16的测定对骨巨细胞瘤的预后判断和治疗方法的选择具有指导意义。

Preliminary Research of Clinical Relevance of MMP-16 and Gaint Cell Tumor

Objective:To study the differences of MMP-16 expression in the gaint cell tumor tissue of recurrent group and non-recurrent group, and then discuss the clinical significance relationship between MMP-16 and relapse status of giant-cell tumor of bone.Methods:First, made collection of specimens from50 patients suffered from the giant cell tumor of bone and divided into recurrent group and non-recurrent group. Second, immunohistochemistry was used to observe the expression levels of MMP-16 in tissues of recurrent group and non-recurrent group. Third, used real-time PCR to measure the expression levels of MMP-16 mRNA. Then, applied western blot to measure the protein levels of MMP-16. Finally, compared the expression levels of MMP-16 between recurrence group and non-recurrence group of giant cell tumor of bone tissues in morphology, gene translation and protein expression, respectively.Results:The results of immunohistochemistry showed that the expression level of MMP-16 in the recurrence group is apparently higher than the non recurrent group. The results of real-time PCR showed that the expression level of MMP-16 mRNA in the recurrent group is apparently higher than the non recurrent group. The results of western blot reveled that the expression level of MMP-16 protein in recurrent group is significantly higher than the non-recurrent group (P<0.05).Conclusion:There is a close relation between MMP-16 and giant cell tumor of bone, the increased expression level of MMP-16 is an important marker of recurrent giant cell tumor of bone. It is guiding significance on the prognosis estimation and the selection of treatment methods to giant cell tumor of bone through the measurement of MMP-16.