传输靶向COX-2 siRNA联合化疗药物对大鼠胃癌细胞生长

目的：通过动物实验探讨传输靶向COX-2 siRNA联合化疗药物对大鼠胃癌细胞生长的抑制作用。方法：24 只健康SD 大鼠 平分为三组，治疗组用COX-2-siRNA转染的胃癌SGC7901 细胞接种，同时进行环磷酰胺、丝裂霉素C 化疗治疗；阴性对照组，用 阴性对照siRNA 转染的胃癌SGC7901 细胞接种，同时进行环磷酰胺、丝裂霉素C 化疗治疗；对照组(n=8)，用未经转染的胃癌 SGC7901 细胞接种，不进行化疗治疗；三组转染后都接种了裸鼠。结果：治疗组、阴性对照组及对照组胃癌细胞凋亡率分别为 （22.28± 0.12）%、（1.23± 0.17）%和（1.03± 0.14）%，治疗组与阴性对照组和对照组比较差异都有统计学意义(t=18.152，17.555， P<0.05)。治疗组的抑瘤率为76.7%，阴性对照组和对照组分别为12.8%和6.89%，治疗组的抑瘤率明显高于其他两组(x2=15. 211，13.899，P<0.05)。Western blotting检测结果显示治疗组的COX-2 蛋白表达含量得到了明显抑制。结论：传输靶向COX-2 siRNA和化疗药物的配合应用可有效抑制COX-2 蛋白的表达，从而抑制胃癌细胞的生长，从而起到更好的治疗效果。

Effects of COX2 siRNA Combined with Chemotherapeutics on the Inhibition of Gastric Cancer Cells in Rats

Objective:To investigate the effects of COX2 siRNA combined with chemotherapeutics on the inhibition of gastric carcinoma cells of rats.Methods:24 healthy SD rats were selected equally divided into three groups, which including the treatment group (n=8) that were given the SGC7901 cells with COX-2-siRNA transfection and treated by the cyclophosphamide and mitomycin C in chemotherapy; the negative control group (n=8) that were given the negative control siRNA transfected cells with gastric cancer SGC7901, and treated by the cyclophosphamide and the mitomycin C in chemotherapy; the control group (n=8) that were given the untransfected SGC7901 without the chemotherapy; Then the mice in the three groups were vaccinated.Results:The rate of the cells'' apoptosis in the three groups was (22.28± 0.12)%, (1.23± 0.17)% and (1.03 ± 0.14)% , respectively with statistically significant differences (t=18.152, 17.555, P<0.05). The rate of antitumor in the three groups was 76.7 %, 12.8% and 6.89%, respectively. The inhibition rate in the treatment group was significantly higher than those of the other two groups (X2=15.211,13.899, P<0.05). Western blotting analysis showed that COX-2 protein expression levels in the treatment group was significantly inhibited.Conclusion:Transmission targeting COX-2 siRNA combined with the chemotherapy drugs can effectively inhibit the expression of COX-2 protein, thereby inhibite the growth of gastric cancer cells, which played better therapeutic effect.