乙肝患者血清的HBV-DNA含量与ALT水平的关系研究

目的:探讨和研究乙型肝炎患者乙型肝炎病毒(HBV)DNA复制水平与丙氨酸氨基转移酶(ALT)水平之间的关系,为临床中乙肝患者的诊治提供参考。方法:对240例慢性乙型肝炎患者采用荧光标记定量PCR方法测定血清HBV-DNA含量,采用全自动生化分析仪测定血清ALT水平,比较和分析HBV-DNA含量与ALT水平之间的关系。结果:慢性乙肝轻、中、重度患者的HBV-DNA含量三组之间差异有统计学意义(p0.01),肝脏的损害程度与HBV-DNA含量之间具有一定的关联,等级相关系数为0.162(P=0.012);ALT水平也与HBV-DNA载量之间存在关联,等级相关系数为0.371(P0.0001)。结论:肝损伤程度与HBV-DNA含量有显著相关性;同时血清ALT水平与HBV-DNA含量呈正相关。检测血清中的HBV-DNA含量和ALT水平为指导乙肝患者HBV感染、复制、传染性的判断、治疗方案的选择和疗效评定提供有一定的依据。

Research of the Relationship between SerumHBV-DNA Content and ALT Level in Hepatitis B Patients

Objective:To discuss and research on the relationship between hepatitis b virus (HBV) DNA content and alanine aminotransferase (ALT) levels of hepatitis b patients, providing reference for the clinical diagnosis and treatment of patients with chronic hepatitis b.Methods:240 cases of patients with chronic hepatitis b were selected, fluorescence labeling quantitative polymerase chain reaction (PCR) were used to detect the serum HBV DNA content, and automatic biochemical analyzer to determine serum ALT level of the patients. The relationship between the HBV-DNA content and ALT level were compared and analyzed.Results:the difference of HBV- DNA content among the three groups (Light, medium and severe chronic hepatitis b patients) all were statistically significant (P<0. 01), liver damage has a certain correlation with HBV-DNA content, and the rank correlation coefficient was 0.162(P = 0.012). ALT has a positive correlation with HBV-DNA loads, the rank correlation coefficient was 0.371 (P<0.0001).Conclusion:Liver damage degree and HBV-DNA content had a significant correlation; At the same time, serum ALT and HBV DNA content were positively correlated. Detection of serum HBV DNA content and ALT level can provide guidance for the evaluation of HBV infection, replication and infectivity, and choice of treatment for hepatitis b patients.