沙棘油对高脂小鼠诱发阿尔兹海默症的预防作用

目的:通过检测沙棘油作用高脂小鼠海马神经元内微管相关蛋白(Tau)及脑源性神经营养因子(BDNF)的表达水平,探讨沙棘油对高脂小鼠并发阿尔兹海默综合征的预防作用。方法:40只KM小鼠,随机取10只为正常对照组;30只以高脂饲料喂养建立高脂模型(HF),按10 mg/kg以生理盐水(阴性对照)、沙棘油(实验)、辛伐他丁(阳性对照)灌胃3 w。取小鼠海马组织进行HE染色、免疫组织化学检测和蛋白印迹分析,检测不同组别小鼠海马神经元内Tau蛋白及BDNF表达的变化。结果:高脂模型组与正常组比较,海马神经元结构在光镜下有明显差别;阴性对照组小鼠海马神经细胞数目减少,神经元内有黄色颗粒样沉淀;实验及阳性组海马损伤有改善,斑块状淀粉样蛋白减少;免疫组化及蛋白印迹显示各组间两种蛋白表达水平不同。结论:沙棘油对高脂小鼠海马体内Tau蛋白表达有抑制作用,加速淀粉样前体蛋白的代谢,降低了由β-淀粉样蛋白沉积诱发阿尔茨海默病的风险;而对BDNF表达有促进作用,能防止神经元受损伤死亡、改善神经元的病理状态、促进受损伤神经元再生。即沙棘油能有效预防高脂人群并发阿尔兹海默综合征。

Preventive Effect of Seabuckthorn Oil on Alzheimer's Disease Induced by Hyperlipidemia Mice

Objective:In order to investigate the prevention effect of seabuckthorn oil on Alzheimer''s disease induced by hyperlipidemia mice, the expression level of microtubule-associated protein (Tau) and brain derived neurotrophic factor (BDNF) of seabuckthorn oil-affected hyperlipidemia mice.Methods:40 KM mice were randomly divided into 2 groups with 10 mice for normal control group and 30 mice for high-fatty model group, the high-fatty model group were adminstrated by gavage once a day for 3 weeks with 10 mg/kg NaCl (negative control group), seabuckthorn oil (experimental group) and simvastatin (positive control group), respectively and with 10 mice for each group, the expression level of Tau and BDNF frommice hippocampus tissues were measured by HE, IHC and WB methods.Results:There was significant difference of the hippocampal neuron between high-fatty model group and normal control group; the mice hippocampus nerve cells decreased in the negative control group and yellow granular precipitated in the neuron; meanwhile, hippocampal damage was improved and the amyloid protein decreased in the experimental and positive control group, IHC and WB results showed that there was significant difference among groups.Conclusion:The above results revealed that seabuckthorn oil could inhibited the expression of Tau of hyperlipidemia mice hippocampus and accelerated metabolism of amyloid precusor protein, so that decrease the risk of beta-amyloid precusor protein deposit-induced Alzheimer''s disease; while seabuckthorn oil could promote the expression of BDNF, so that prevent injury and death of neurons, improve pathological state of neurons and promote regeneration of injured neurons, indicating seabuckthorn oil could effective prevent hyperlipidemia people concurrent Alzheimer''s disease.