表皮生长因子诱导骨髓间充质干细胞向视网膜神经细胞分化的体外研究

目的:研究表皮生长因子诱导骨髓间充质干细胞向视网膜神经细胞分化的可能性。方法:体外培养骨髓间充质干细胞,利用流式细胞仪分析其细胞表型。采用含EGF的培养液诱导骨髓间充质干细胞向视网膜神经细胞分化,并利用免疫荧光法进行鉴定。结果:从骨髓中分离培养的细胞具有成纤维细胞样形态,贴壁生长,表型相对均一,表面标志为CD90、CD44、CD147阳性;而CD34、CD38、CD45、CD14、HLA-DR阴性。体外诱导后可以得到神经干细胞标志物nestin、神经胶质细胞标志物GFAP和视网膜光感受器细胞标志物Rhodopsin呈阳性表达的细胞。结论:从骨髓中分离培养得到的间充质干细胞具有向视网膜神经细胞分化的潜能。

Experimental Research on the Differentiation of Human Bone Mesenchymal StemCells into Retinal Cells in vitro

Objective:To study the differentiation of human bone marrow-derived mesenchymal stemcells into retinal cells in vitro.Methods:HMSCs were isolated fromhuman bone marrow after Ficoll density gradient centrifugation. The adherent cells after cultivation at least 3 passages were used for study. Immunophenotype of the cells was analyzed by Flow cytometer, and cellular differentiation was identified by immunofluorescence labeling technique.Results:The target cells derived from human bone marrow adhered to the plate with fibroblastic-like morphology, whose surface markers were similar as mesenchymal stem cells. Major cells were positive for CD90, CD44 and CD147, while they were all negative for CD34, CD45 and HLA-DR. In the differentiation study, HMSCs were cultured in induced medium can differentiate into nestin positive cell, GFAP positive glial cells and retina-specific neurons expressing Rhodopsin with CD90 negative.Conclusion:HMSCs are capable of differentiating into the retinal neural cells in vitro.