成年小鼠血液系统对亚磁场的响应

目的:研究亚磁场对成年小鼠血液系统的影响。方法:将成年雄性C57BL/6小鼠(4-6周,20±2g,n20,每笼4只)随机分组,分别饲养在模拟亚磁场环境(500nT)和对照地磁场环境(~50μT)。每周定时监测动物体重变化和饮食消耗两次。一个月后,采集亚磁处理小鼠和地磁对照小鼠全血和血清样品,分别进行血常规监测和血清微量元素分析。同时检测血清中过氧化氢(H2O2)的含量,以及超氧化物歧酶(SOD)和过氧化氢酶(CAT)的活力。结果:亚磁场处理过程中,动物体重和饮食消耗与地磁对照没有显著差异,但是体重增量在2周后(14天-24天)比对照组有显著降低(P0.05)。一个月亚磁场处理后,红细胞,血小板和总白细胞处于正常水平,没有发生显著变化,但是中性粒细胞水平显著上升(P0.05)。血清中微量元素水平和氧化应激指标没有显著变化。结论:成年小鼠在亚磁场中经历了一定程度的适应反应。经过一个月连续亚磁场处理,血液系统能够维持健康水平,但是嗜中性粒细胞对亚磁场存在明显响应。

The Hematopoietic SystemResponses to One-Month Continuous Hypomagnetic Field Exposure in Adult Mice

Objective:To investigate the effects of the hypomagnetic field (HMF) on the hematopoietic system of adult mice.Methods:Adult male C57BL/6 mice (4-6 weeks old, 20± 2g, n>20, 4 mice per cage) were reared in the simulated HMF condition (< 500 nT) and the geomagnetic field (GMF) control environment (~50 uT). The body weight change and food and drink uptakes were monitored twice every week. Whole blood and serum samples of the HMF-exposed and GMF control mice were collected and sent for blood cell analysis and blood microelement assay, respectively. The levels of hydrogen peroxide (H2O2) and the activity of superoxide dismutase (SOD) and catalase (CAT) in the serumwere measured.Results:Body weight of the HMF-exposed mice was identical with the control animals during the one-month exposure period. But the body weight increase of the HMF-exposed mice was lower than the control from day 14 to day 24 (P<0.05). Blood routine analysis showed that the indexes of red blood cell, platelet and total white blood cells of the HMF-exposed and GMF control mice were the same, both in the healthy range. But the concentration of neutrophil granulocyte was significantly increased after one-month HMF exposure (P<0.05). However, no significant change was observed in serum microelements and the oxidative stress indexes.Conclusion:The adaptation response of adult mice to short-term HMF exposure was observed. After one-month HMF exposure, the hematopoietic system was under the healthy condition except that the response of neutrophils to the HMF was observed.