细胞色素P450催化二甲基亚硝胺C-H键羟基化机理的理论研究进展

细胞色素P450是广泛存在于哺乳动物微粒体和线粒体内的一类亚铁血红素—硫醇盐蛋白的超家族。它参与内源性物质和包括药物、环境化合物在内的外源性物质的代谢。其代谢机理引起人们的极大关注,同时也存在诸多挑战。通过对不同底物代谢机理的研究有助于人们深入认识P450的结构及其催化机理,还可以为物质的体内代谢提供理论指导。本文主要对P450的催化氧化机制,二甲基亚硝胺在细胞色素P450作用下的代谢机理研究进展及P450的活性氧化物等方面的研究进行了综述。

Progresses of NDMA C-H Bond Hydroxylation Mechanisms Catalyzed by Cytochrome P450

Cytochrome P450 was a class of heme-thiolate protein superfamily, which widely found in microsomes and mitochondria of living mammalian. It can catalyze the metabolism reaction, includes endogenous compounds and also exogenous compounds like medicine and environment chemicals. The mechanisms has driven a great deal of attention, while there are still many challenges. Research on NDMA C-H metabolism mechanism will help people deeply understand the structure of P450 and its catalytic mechanism. In this paper the catalytic mechanism of P450, the catalysed hydroxylation mechanism of N, N-dimethylnitrosamine and the reactive oxygen species are reviewed.