变应性鼻炎模型小鼠鼻黏膜的形态学观察

目的:建立小鼠变应性鼻炎模型,观察小鼠鼻腔黏膜组织的重塑情况。方法:20只BALB/c小鼠被随机分为致敏组和对照组,使用卵清蛋白(OVA)诱导建立小鼠变应性鼻炎模型。通过HE染色观察小鼠鼻黏膜的大体重塑情况,吉姆萨染色观察嗜酸性粒细胞,阿辛蓝-过碘酸-希夫染色观察杯状细胞;酶联免疫吸附(ELISA)法检测小鼠血清中白细胞介素-4(IL-4)的水平。结果:小鼠变应性鼻炎模型的生物学行为评分为6.5±1.3,提示造模成功。与对照组相比,致敏组鼻腔黏膜出现上皮细胞脱落、坏死,杯状细胞增生,鳞状上皮化生,固有层和黏膜下层腺体增生、血管扩张,组织水肿,固有层内可见特征性的嗜酸性粒细胞浸润,造模后鼻腔黏膜结构存在重塑。致敏组小鼠鼻黏膜嗜酸性粒细胞计数及杯状细胞计数分别为(26.4±5.72)和(24.14±3.12),而对照组分别是(8.31±2.42)和(9.41±1.22),两组比较均具有统计学差异(P0.05);致敏组血清中白细胞介素4(IL-4)水平为(18.9±3.1)pg/ml,对照组为(8.3±1.4)pg/ml,致敏组显著高于对照组,差异有统计学意义(P0.05)。结论:通过卵清蛋白诱导建立的小鼠变应性鼻炎模型鼻腔黏膜存在组织重塑。

Observation on the Nasal Mucosa Remodeling in Mouse Model of Allergic Rhinitis

Objective:To observe the nasal tissue remodeling in a mouse model of allergic rhinitis.Methods:Twenty BALB/c mice were randomly divided into the allergic rhinitis group and control group. Ovalbumin (OVA) was used to establish the mouse allergic rhinitis model. Hematoxylin-eosin (HE) staining, Giemsa staining and Alcianblue-periodicacid-Schiff staining were carried out to observe the tissue remodeling, eosinophils and goblet cells, respectively. Enzyme linked immunosorbent assay (ELISA) was used to detect the seruminterleukin 4 (IL-4) level.Results:The mouse allergic rhinitis model was successfully established, the score of biological behaviour was (6.5± 1.3). Structural remodeling occured in nasal mucosa of allergic rhinitis group. Compared with the control group, there were obviously cell desquamation, necrosis, squamous metaplasia and goblet cell hyperplasia in epithelial layer. In the lamina propria and submucosa, glandular hyperplasia, vasodilatation, edema and characteristic eosinophils infiltration were also found in the allergic rhinitis group. The eosinophils and goblet cell counts were 26.4± 5.72 and 24.14± 3.12 in allergic rhinitis group respectively, which were 8.31± 2.42 and 9.41± 1.22 in the control group respectively(P<0.05). The serum IL-4 level was (18.9± 3.1)pg/ml in allergic rhinitis group and (8.3± 1.4)pg/ml in control group, which was significantly higher in allergic rhinitis group than that of the control group (P<0.05).Conclusion:Structural remodeling occured in the nasal mucosa of allergic rhinitis mouse induced by OVA.