腋臭患者大汗腺中ApoD和AR的表达水平与气味强度间的关系

目的:明确大汗腺组织中ApoD和AR的表达水平与腋臭患者散发出气味间的关系,阐明两者在腋臭发病中的作用,为腋臭的临床治疗和干预奠定基础。方法:收集在我院整形外科就诊的腋臭病人腋下皮肤组织标本41例。手术前由3名医生共同判断患者腋下气味强度,按照能在≤1 m、≤3 m、≤5 m距离闻及气味把患者分为轻、中、重三组。随机抽取各组标本组织块进行RT-PCR检测,测定ApoD和AR在大汗腺组织中的含量。结果:根据气味强度判定标准,轻、中和重3组患者人数分别为:7人、11人和23人。按照AB 7500 Real-Time PCR系统分析结果所示ApoD相对表达量的等级与气味强度之间有线性关系(P0.05),ApoD相对表达量的等级随着气味强度的增加而升高;AR相对表达量的等级与气味强度之间有线性关系(P0.05),随着气味强度的增加而升高。结论:ApoD和AR的表达量与腋臭气味强度之间有着密切的联系。进一步证实AR可调节大汗腺组织ApoD的表达,为明确腋臭的发病机制提供了理论依据。

Relationship between ApoD and AR in the Apocrine Sweat Glands and Odor Intensity in Axillary Osmidrosis Patients

Objective:To study the relation of ApoD/AR and odor strength in apocrine sweat gland of axillary osmidrosis and to confirmthe effect in the pathogenesis of axillary osmidrosis so as to establish the theoritical basis for the pathogenesis of disease.Methods:We collected armpit skin tissue 41 samples of axillary osmidrosis group in our hospital. Three doctors smell armpit odor in different distances( 1 m, 3m and 5 m). The extent of the osmidrosis were classified into mild, middle and severe degree correspondent to the unpleasant odor obviously smelled at 1, 3 and 5 meters. Then we detected the content of ApoD and AR through Real-time PCR. Statistical analysis and processing were done later.Results:There were 7 patients in mild degree, 11 patients in middle degree and 23 patients in severe degrees. The Real-Time PCR showed that there was relationship between the degree of relative expression of ApoD/AR and odor strength(P<0.05). The degree of relative expression of ApoD increased with the increase of odor strength.Conclusion:There was a direct relationship between the degree of relative expression of ApoD/AR and odor strength. It may play a crucial role in the process of axillary osmidrosis odor that should be confirmed in the future.