血清应答因子在食管鳞癌细胞侵袭转移中的作用

目的:研究血清应答因子(serum response factor,SRF)在人食管鳞癌细胞体外侵袭转移中的意义。方法:选用EC9706-H、EC9706-L和EC109-H、EC109-L两对高低转移细胞系,采用细胞划痕实验验证食管鳞癌高低转移细胞系体外侵袭转移能力的差异;Western blot检测SRF在两对食管鳞癌高低转移细胞系中的差异表达;在EC9706-H、EC109-H细胞中加入CCG(SRF抑制剂)抑制SRF的表达后,检测其侵袭转移能力的变化。结果:细胞划痕实验验证了两对食管鳞癌高低转移细胞系侵袭转移能力的差异;Western blot结果提示SRF在EC9706-H、EC109-H细胞中的表达水平显著高于EC9706-L、EC109-L细胞;在EC9706-H、EC109-H细胞中加入CCG抑制SRF的表达后,其侵袭转移能力明显减退。结论:SRF在高转移性食管鳞癌细胞系中呈现高表达,在低转移性食管鳞癌细胞系中呈现低表达,抑制高转移性食管鳞癌细胞系中SRF的表达后,其侵袭转移能力下降,提示SRF和食管鳞癌的侵袭转移能力呈正相关。

The Effect of SRF on the Invasion and Metastasis of Human Esophageal Squamous Cell Carcinoma

Objective:The aimof this study was to investigate the in vitro effect of serum response factor (SRF) on the invasion and migration of esophageal squamous cell carcinoma (ESCC) in vitro.Methods:The invasion and migration of ESCC cell lines were detected by scratch assays. The expression of SRF in ESCC cell lines were measured by western blot. CCG was used to suppress the expression of SRF in highly invasive cells of ESCC.Results:There were differences with the invasion and migration in the ESCC cell lines. The expression of SRF in highly invasive cells of ESCC was higher than the matched lowly invasive cells. After treatment with CCG, the invasion and migration of highly invasive cells were obviously decreased.Conclusion:The expression level of SRF may be positively correlated to the invasion and migration of ESCC cell lines, and SRF may be a novel molecular marker for the prediction of migration, diagnosis and therapy of ESCC.