Treg细胞在慢性乙肝患者外周血中的表达及其对DC细胞的抑制作用

目的:检测慢性乙型肝炎患者外周血中Treg细胞的比例,探讨其对DC细胞的免疫抑制作用。方法:检测CHB患者和正常对照组外周血CD4+,CD25+,Treg及DC表面因子CD80和HLA-DR的表达;在DC培养不同时间内加入不同比例CHB Treg细胞,观察DC对淋巴细胞增殖的影响以及细胞因子IL-10及TGF-β的表达变化。结果:CHB患者外周血Treg细胞比例高于对照组,差异有统计学意义(P0.05);CHB患者DC细胞表面分子CD80和HLA-DR表达均低于对照组,差异有统计学意义(P0.05);经Treg细胞处理后,DC细胞刺激淋巴细胞的增殖能力下降,淋巴细胞增殖的抑制率显著升高(P0.05);随Treg细胞增加,IL-10及TGF-β水平升高,差异有统计学意义(P0.05)。结论:CHB患者外周血中Treg细胞的增加可能通过诱导IL-10和TGF-β的表达来抑制DC细胞的免疫功能。

Immunosuppression of Treg on the Regulation of Dendritic Cells in Patients with Chronic Hepatitis B

Objective:To investigate the Treg cells suppressing the dendritic cells in the pathogenesis of chronic hepatitis B.Methods:CD4+CD25+ regulatory T cells and the expression of CD80 and HLA-DR on DC in patients with CHB and the control group were detected. Treg cells of CHB and DC of the controls were cultured with different ratio and time, then the concentrations of IL-10 and TGF-beta were detected by ELISA.Results:The frequency of Treg cells in CHB group was higher than that of the control group with statistically significant difference (P<0.05); The expressions of CD80 and HLA-DR in CHB group were lower than those of the control group with statistically significant difference (P<0.05); Then CD4+ CD25+ regulatory T cells in CHB group could significantly suppress effects on DC immunity with time-and-dose depended; The secretion of IL-10 and TGF-beta increased significant in CHB group(P<0.05).Conclusion:The increasing Treg cells of patients with CHB may suppress DC by inducing the expressions of IL-10 and TGF-beta.