仙灵骨葆对骨质疏松大鼠OPG/RANK/RANKL 表达的影响

目的:观察仙灵骨葆治疗骨质疏松模型大鼠后,对大鼠体内OPG/RANKL/RANK表达的影响。方法:卵巢摘除法建立SD大鼠骨质疏松模型,设立假手术组、对照组(单纯去卵巢组)、雌激素组(给予17β-雌二醇)和治疗组(给予仙灵骨葆)。术后1周开始给药,给药12周后检测各组大鼠股骨骨密度,ELISA法检测血清中OPG/RANKL含量,RT-PCR检测骨组织中OPG/RANK/RAN-KL mRNA表达,免疫组化检测骨组织中RANK的表达。结果:对照组大鼠骨密度显著低于假手术组;治疗组和雌激素组大鼠O-PG表达显著高于对照组,RANK及RANKL的表达显著低于对照组。结论:采用卵巢摘除法成功建立大鼠骨质疏松模型;仙灵骨葆可促进骨质疏松大鼠OPG的表达,并抑制RANK及RANKL的表达,对骨质疏松模型大鼠有治疗作用。

Effect of Xianlinggubao on the Expression of OPG/RANK/RANKL in Osteoporotic Rats

Objective:To investigate the impact of Xianlinggubao treatment on OPG/RANKL/RANK expression in osteoporosis rats.Methods: SD rat model of osteoporosis was established by ovariectomy and divided into four groups: the sham operation group,the control group(OVX simply),the estrogen group(given 17β-estradiol) and treatment group(given Xianlinggubao).The rats were administered one week after surgery.After 12 weeks dosing,all rats had their bone mineral density detected.The OPG/RANKL content in serum were detected by ELISA,and the OPG/RANKL/RANK mRNA expression in bone tissue were detected by RT-PCR,and the RANK expression in bone tissue were detected by immunohistochemical assay.Results: The bone mineral density in control group was significantly lower than the sham operation group;The OPG expression in treatment/estrogen group were significantly higher than the control group;The RANK and RANKL expression in treatment/estrogen group were significantly lower than the control group.Conclusion: A rat model of osteoporosis was successfully established by the ovariectomy method;Xianlinggubao can promote the expression of OPG and inhibit the expression of RANK/RANKL in osteoporosis rats.It has a therapeutic role in the rat model of osteoporosis.