探讨尿视黄醇结合蛋白对高血压早期肾损害的作用研究

目的:探讨尿视黄醇结合蛋白(RBP)、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)、尿胱蛋白酶抑制剂C(Cystatin C)的测定对原发性高血压早期肾损害诊断的敏感性及临床意义.方法:选择2011年4月～2011年11月在新疆自治区人民医院肾病科住院的原发性高血压患者69例作为观察组,另设健康体检者30例为对照组.尿RBP、Cystatin C测定采用酶联免疫法(ELISA)检测,尿NAG测定采用对硝基苯酚(PNP)比色法检测.结果:观察组中尿RBP、NAG、CystatinC的测定含量明显高于正常对照组(P＜0.001),通过ROC曲线、诊断试验结果显示:尿RBP的曲线下面积为0.962,95％可信区间为0.921～0.983,较尿NAG、Cystatin C更具有敏感性(P＜0.001).结论:检测尿RBP有助于高血压肾损害的早期监测,对预防高血压性肾病的发生、发展具有重要的临床价值.

Explore Urine Retinol Combined with Protein to High Blood Pressure Early Renal Damage Role Research

Objective: The sensitive index and clinical significance in early renal injury caused by primary hypertension was inves-tigate by detecting Urine retinol combined with protein(RBP),N-acetyl-?茁-D-amino grape glycosidase(NAG),urine micro proteinase in-hibitor C(Cystatin C).Methods: 69 patients with primary hypertension were enrolled who were in-patients,dated from April 2011 to November 2011;30 subjects of healthy people for the outpatient were selected as control group.The urine RBP and urine Cystatin C were respectively determined by enzyme linked immunosorbent assay(ELISA).Urine NAG was measured by p-nitrophenol(PNP) col-orimetric assay.Results: In case group urine RBP,NAG and the Cystatin C determination content were higher that in the normal control group,the differences were statistically significant(P<0.001).Through the ROC curve and diagnostic test,the area under the curve of Urine RBP were 0.962,the 95% confidence interval were 0.921～0.983(P<0.001).Conclusions: The urine RBP could be helpful in early detection of hypertensive renel injury,and there was important clinical value in the prevention of hypertensive renel injury.