蝉蜕、僵蚕对大鼠系膜增生性肾炎作用的实验研究

目的:研究蝉蜕、僵蚕对大鼠系膜增生性肾小球肾炎(MsPGN)的治疗作用。方法:采用改良慢性血清病法制备的MsPGN模型,随机分为模型对照组、蝉蜕高剂量组、蝉蜕低剂量组、僵蚕高剂量组、僵蚕低剂量组,另设正常对照组。分别在用药5周、8周后检测大鼠24h尿蛋白;8周后处死大鼠,行血液生化指标检测和肾组织HE染色观察,免疫组化法检测TGF-β1的表达水平。结果:与模型对照组比较,用药5周后蝉蜕、僵蚕高剂量组显著降低大鼠24h蛋白尿(P<0.01);8周后各治疗组大鼠肾组织TGF-β1表达量,24h蛋白尿和血清胆固醇均显著下降,蝉蜕高、低剂量组血清白蛋白均有所升高,差异具有显著性意义(P<0.01)。肾组织形态学观察显示,蝉蜕、僵蚕高剂量组个别区域肾小球系膜细胞轻度增生,系膜区轻度增宽,管腔无挤压现象,较模型组明显改善。结论:蝉蜕、僵蚕均能有效降低MsPGN大鼠24h尿蛋白,改善脂质代谢,其作用机制可能与抑制TGF-β1的过度表达有关。

Experimental Study of Periostracum Cicadae and Bombyx Batryticatus in the Treatment of Mesangial Proliferative Glomerulonephritis of Rats*

Objective: To investigate the effects of periostracum cicadae and bombyx batryticatus on the treatment of mesangial proliferative glomerulonephritis(MsPGN) of Rats,and to explore its mechanism.Methods: The models of MsPGN rats were established by ameliorated chronic disease serum methods.Rats were randomly divided into model control group,high periostracum cicadae group,low periostracum cicadae group,high bombyx batryticatus group,low bombyx batryticatus group,and normal control group.Five weeks and eight weeks later,urinary protein were detected.Pathological changes were detected by hematoxylin-eosin staining(HE),and the expression levels of TGF-β1 were detected by immunohistological method.Results: Compared with that in the control group,24h urinary protein decreased significantly in both high groups after five weeks(P<0.05),and the levels of expressions of TGF-β1,24h urinary protein and serum cholesterol decreased significantly in every drugs groups,and serum albumin increased significantly in high and low periostracum cicadae group(P<0.05)after eight weeks.There was no significant difference between high and low groups about the levels of expressions of TGF-β1.Conclusions: Periostracum cicadae and bombyx batryticatus may decrease 24h urinary protein and improve lipid metabolism in MsPGN rats,and it may be related with the inhibit of TGF-β1 over-expressiom.