| TUNEL 法检测抑郁模型大鼠海马细胞凋亡的改进及应用 |
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| 引用本文： | 王发渭,黄泉智,许成勇,孙志高,陈利平,胡亚卓,韩志涛.TUNEL 法检测抑郁模型大鼠海马细胞凋亡的改进及应用[J].现代生物医学进展,2012,12(17):3221-3223. |
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| 作者姓名： | 王发渭 黄泉智 许成勇 孙志高 陈利平 胡亚卓 韩志涛 |
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| 作者单位： | 1. 解放军总医院中医科 北京100853
2. 解放军总医院老年医学研究所病理生理研究室 北京100853 |
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| 基金项目： | 北京市科技计划基金课题(Z090507017709030) |
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| 摘 要： | 目的:探讨TUNEL法检测抑郁模型大鼠海马神经元细胞凋亡的改良方法。方法:20只SD大鼠随机分为空白对照组、模型组。复制孤养加慢性轻度不可预见性的应激抑郁模型(21天)。分别采用TUNEL试剂盒标准法和改良法观察大鼠海马区凋亡细胞的变化。结果:改良法与试剂盒标准法比较,非特异性染色明显减轻,背景清晰,差异明显。结论:改良的TUNEL法检测海马神经元细胞凋亡结果更真实可靠。
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| 关 键 词： | 抑郁模型 海马 细胞凋亡 TUNEL |
Application Experience of TUNEL in Detecting Hippocampus
Apoptosis of Depression Rats |
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| WANG Fa-wei,HUANG Quan-zhi,XU Cheng-yong,SUN Zhi-gao,CHEN Li-ping,HU Ya-zhuo,HAN Zhi-tao.Application Experience of TUNEL in Detecting Hippocampus
Apoptosis of Depression Rats[J].Progress in Modern Biomedicine,2012,12(17):3221-3223. |
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| Authors: | WANG Fa-wei HUANG Quan-zhi XU Cheng-yong SUN Zhi-gao CHEN Li-ping HU Ya-zhuo HAN Zhi-tao |
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| Institution: | 1 Department of Traditional Chinese Medicine,Chinese PLA General Hospital Beijing,100853,China; 2 Institute of Geriatrics,Chinese PLA General Hospital Beijing,100853,China) |
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| Abstract: | Objective: To investigate the method to detect the hippocampus apoptosis of chronic stress depression rats.Methods: 20 SD rats were randomly divided into control group and model group.The model group was given the chronic unpredicted mild stress for 21 days to induce depression.The hippocampus apoptosis was detected by the TUNEL kit method and the improvement method.Results: Compared with TUNEL kit method,the improvement method avoided false positive reactivity,and a clear background was observed.Conclusion: The improvement TUNEL method is a better way to detect the hippocampus apoptosis of chronic stress depression rats. |
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| Keywords: | Depression model Hippocampus Apoptosis TUNEL |
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