脐血干细胞移植治疗肝硬化的临床研究

目的:观察脐血干细胞移植治疗失代偿期肝硬化的临床疗效。方法:对60例失代偿期肝硬化患者进行自身对照的临床研究。在无菌条件下取健康产妇足月生产的脐带血,分离纯化脐血干细胞,通过肝动脉途径,将纯化的脐血干细胞移植入患者肝脏内,于移植后2周、4周、8周进行肝功能、凝血指标检测,并于4周及8周行腹部B超及胃镜检查,观察患者移植后不同时间症状改善情况及术后不良反应的发生情况。结果:60例接受脐血干细胞移植的肝硬化患者术中术后无明显不良反应发生。移植后,患者临床症状改善明显,食欲不振、乏力、腹水等减轻甚至消失;血清学检测:白蛋白水平较术前明显升高,凝血酶原时间、总胆红素较术前明显下降;术后8周复查胃镜,食道静脉曲张没有明显变化。结论:脐血干细胞移植治疗失代偿期肝硬化是一种安全、有效的方法,尤其是在提高白蛋白水平及改善凝血功能方面有很好的疗效,可作为肝移植治疗的过渡或补充治疗。

Clinical Observation of the effect of Umbilical Cord Blood Stem Cell Transplantation on Patients with Decompensated Cirrhosis

Objective: To investigate the clinical therapeutic effect of umbilical blood stem cell transplantation on patients with de-compensated liver cirrhosis.Methods: By using self-controlled,60 cases of patients with decompensated cirrhosis were studied.Umbilical cord blood was collected from mature fetuses delivered by healthy parturient women under sterile conditions.Stem cells were separated and were transplanted into a patient’s liver through the hepatic arteries.At different time(2,4 and 8 wk) after transplantation,liver func-tion and coagulation index tests were performed.Abdominal ultrasounds and gastroscopes were obtained 4 and 8 wk after the operation.Changes and adverse reactions in the 60 patients were observed.Results: No remarkable side effect was discovered in all 60 patients.Their clinical symptoms,such as poor appetite,physical weakness,and peritoneal dropsy were either relieved or entirely disappeared.Al-bumin levels increased and total bilirubin remarkably decreased.No evident change took place in the esophagus varix based on the gas-troscopy performed 8 wk post-transplant.Conclusions: CBSCT is a safe and effective therapy for decompensated hepatocirrhosis.Albu-min levels and liver coagulation capacity are improved.CBSCT can serve as an alternative or a supplement to liver transplantation.