骨诱导无机材料BAM 修复牙种植体周围骨缺损的实验研究

目的:评价骨诱导磷酸钙生物陶瓷(BAMOICPC)与可吸收胶原膜(BME-10X医用胶原膜)在牙种植体周围骨缺损中的修复能力。方法:在兔股骨上植入羟基磷灰石涂层BLB种植体,然后在其侧壁制造高4 mm、宽3 mm、深2 mm的骨缺损。对照组为单纯侧壁骨缺损,实验A组骨缺损区仅覆盖BME-10X膜,B组骨缺损区植入BAMOICPC,C组骨缺损区植入BAMOICPC并加盖BME-10X膜。于术后6个月取带种植体的骨段,通过HE染色和扫描电镜(SEM)分析。结果:对照组骨缺损区种植体表面见纤维包裹,实验A组骨缺损边界区少许骨质移行覆盖,实验B组下半部分缺损区新生骨覆盖。C组新生骨完全覆盖骨缺损区,且较B组硬度高,扫描电镜见与种植体结合更紧密。组织学观察B、C两实验组新生骨均可见比较成熟的哈弗氏管系统。结论:骨诱导磷酸钙生物陶瓷BAMOICPC是一种较理想的骨替代材料,联合运用胶原膜修复种植体周骨缺损效果佳。

Experimental Study about BAM Osteoinduction Calcium Phosphate Ceramic in Repairing the Bone Defect around Dental Implant

Objective: To evaluate the ability of BAM osteoinductive calcium phosphate ceramic(BAMOICPC) with absorbable collagen membrane(BME-10X medical collagen membrane) in repairing the bone defect around dental implant.Methods: BLB implants coated by hydroxyapatite(HA) were implanted in femur of rabbit.Standard opening bone defect on one side of implant(4mm in height,3mm in width,2mm in thickness)was made.In the control group,the side wall bone defect was open.In the experimental group A,the side wall bone defect was covered simply by BME-10X collagen membrane.In the experimental group B,BAMOICPC were implanted on the bone defects.In the experimental group C,BAMOICPC were implanted on the bone defects and covered with BME-10X collagen membrane.On the 6th postoperative month,animals were killed and sampled.The bone with the implant segment were analyzed by HE staining and scanning electron microscopy(SEM).Results: In the control group,the surface of implants in the area of bone defect were wrapped by fibers.In group A,a little bone covered the border of bone defect.The experimental bone defects in group B were partially covered by new bone.In group C,the bone defects were completely covered by new bone.Compared with group B,the new bone in group C was high hardness,and combined with the implant more tightly by SEM observation.The mature haversian canals system were observed both in B and C experimental groups by histology methods.Conclusion: BAM osteoinductive calcium phosphate ceramics(BAMOICPC) is an ideal bone substitute material.Combined with collagen membrane,BAMOICPC repaired the bone defect around dental implants more excellently.