大肠杆菌变种的两种分子生物学检测方法分析

目的:对大肠杆菌的一种重要的变种--肠出血性大肠杆菌O157-H7的几种检测方法进行比较研究.方法:以自动免疫磁珠收集系统(AIMS)、自动酶标免疫测试系统(VIDAS)与传统常规的分离方法进行对比分析.结果:运用自动免疫磁珠收集系统(AIMS)方法对80份可能含有肠出血性大肠杆菌O157-H7的实样进行检测,检出份数为6份,检出率为7.5％,而且在一周之内可以全部对上述检出实样进行鉴定.AIMS法能够检出浓度在10cfu/mL模拟实样之中的肠出血性大肠杆菌O157-H7,然后将此法与CHROMagar 0157琼脂板相结合,其效果则更为明显.而自动酶标免疫测试系统(VIDAS)与传统与的分离方法则检测的效果不佳,检出率为0.自动免疫磁珠收集系统(AIMS)检测方法与自动酶标免疫测试系统(VIDAS)、传统与的分离方法在检出率方面存在显著的统计学差异,P＜0.01.结论:运用自动免疫磁珠收集系统(AIMS)结合CHROMagar 0157琼脂板对出血性大肠杆菌O157-H7进行检测,检出率较高、灵敏度较高且快速便捷,可以用于O157-H7外环境检测与食品污染源的实际调查之中,应该对其加以广泛地推广并应用.

Analysis of Escherichia Coli Variants of the Molecular Biology Detection Methods

Objective: Escherichia coli is a kind of important variants——enterohemorrhagic Escherichia coli O157-H7 several detection methods are comparative study.Methods: The automated immunomagnetic beads collection system(AIMS),automatic enzyme immunoassay test system(VIDAS) and traditional and separation method for comparative analysis.Results: The use of automated immunomagnetic beads collection system(AIMS) on80may contain enterohemorrhagic Escherichia coli O157-H7samples for detection,detection of copy number is 6,the detection rate was 7.5%,and within a week to all of the above can be detected samples for identification.AIMS method can detect concentration at 10cfu / mL simulation samples of enterohemorrhagic Escherichia coli O157-H7,then this method and CHROMagar 0157agar plate combination,the effect is more obvious.Automatic enzyme immunoassay test system(VIDAS) and traditional and separation method detection effect is poor,the detection rate is 0.Conclusion: The use of automated immunomagnetic beads collection system(AIMS) combined with CHROMagar0157agar plates for hemorrhagic Escherichia coli O157-H7were detected,the detection rate is higher,higher sensitivity and fast and convenient,can be used in O157-H7environment detection and food contamination sources of practical investigation,should be widely promoted and applied.