粪肠球菌生物被膜形成早期相关基因的表达研究

目的:以粪肠球菌为研究对象,探讨粪肠球菌基因srtA(转肽酶A编码基因)、esp(肠球菌表面蛋白)与粪肠球菌生物被膜形成早期的相关性。方法:用逆转录PCR与实时荧光定量PCR方法对生物被膜和浮游菌组细菌srtA、esp两种与生物被膜形成早期相关的基因其表达进行检测,并进行统计学分析。结果:srtA、esp基因与粪肠球生物被膜菌早期形成密切相关。生物被膜菌组srtA、esp表达量分别是浮游菌组的7.9与13.5倍。结论:srtA、esp基因与粪肠球生物被膜菌形成早期密切相关,可能是生物被膜早期形成的上调因子。

Expression of Genes for Early Biofilm Formation in Enterococcus Faecalis

Objective: To study the relationship between the expression of srtA and esp and early biofilm formation in Enterococcus faecalis.Methods: RT-PCR and real-time quantitative PCR method for biofilm and planktonic Enterococcus faecalis groups of srtA and esp,two kinds of early biofilm formation genes whose expression were detected and analyzed statistically.Results: The srtA and esp are colsely related to the early biofilm formation in Enterococcus faecalis.The expression quantity of srtA and esp in biofilm group is 7.9 times and 13.5 times respectively of planktonic group.Conclusions: The srtA and esp are colsely related to the biofilm formation,which promote the early biofilm formation in Enterococcus faecalis probably.