雷公藤甲素对结肠癌细胞SW480 的基因表达谱的影响

目的:探讨雷公藤甲素对结肠癌SW480细胞的基因表达谱的影响。方法:雷公藤甲素处理结肠癌SW480细胞24h后,分别提取给药组和空白对照组SW480细胞总RNA,纯化并逆转录成用Cy3和Cy5标记的cDNA探针,经全基因芯片杂交,洗涤,通过生物信息学方法分析雷公藤甲素处理组和空白对照组SW480细胞基因表达谱的差异。结果:与空白对照组比较,共发现了902个差异基因,雷公藤甲素处理组有196个基因上调,706个基因下调。上调基因主要涉及细胞代谢。下调基因主要涉及wnt通路、细胞周期通路、Toll样受体通路以及MAPK等通路。结论:雷公藤甲素能导致结肠癌细胞基因表达谱的改变,这些基因改变可能参与了细胞增殖、分化、凋亡等过程。这些信息可能为探讨雷公藤抗结肠癌作用机制提供线索。

Effect of Triptolide on Gene Expression Profile of Colon Cancer Cells SW480

Objective: To investigate the effect of triptolide on the gene expression of human colon cancer SW480 cells.Methods: incubate SW480 cells with or without triptolide for 24 hours,and then the total RNAs were extracted.All RNA were purified and then reversely transcribed to cDNA.The cDNAs were tagged with fluorescence dye Cy3/Cy5,and then hybridized with whole-genome chip.After washing,the gene expression profile of SW480 cells was analyzed by bioinformatics to screen the differential expressed genes among the cells with or without triptolide.Results: Compared with the control group,a total of 902 differentail genes in triptolide-treated group were found,among which,196 were up-regulated and 706 were down-regulated.The up-regulated genes were mainly involved in metabolism,and down-regulated genes mainly involved in Wnt signaling pathway,cell cycle pathway,Toll-like pathway and MAPK pathwayand so on.Conclusion: Triptolide alters the gene expression profiles of colon cancer cells SW480,and these genes are mainly re-lated to cell proliferation,differentiation,and apoptosis.The screening of the genes may be meaningful for study the mechanisms of trip-tolide treat colon cancer.