三氧化二砷对人脐静脉内皮细胞凋亡及VCAM-1/ICAM-1 表达的影响

目的:观察三氧化二砷(As2O3)对血管内皮细胞增殖、凋亡及VCAM-1/ICAM-1表达的影响,探讨As2O3对血管内皮细胞增殖生长以及炎症反应的影响。方法:人脐静脉内皮细胞(HUVEC)体外培养,以不同As2O3浓度及时间对其进行干预。采用CCK-8测定细胞增殖活性,流式细胞仪AnnexinⅤ/PI双染法检测细胞的凋亡率,实时荧光定量PCR检测VCAM-1mRNA表达,酶联免疫吸附试验(ELISA)检测细胞间黏附分子(VCAM-1)及血管细胞黏附分子(ICAM-1)的表达情况。结果:当As2O3浓度在3μmol.L-1时HUVEC培养24 h的的凋亡率为(0.134±0.03)%,48 h为(3.305±0.53)%,72 h为(3.748±0.84)%(P<0.05),凋亡率均在一较低水平。当As2O3浓度>3μmol.L-1时HUVEC凋亡率明显增加(P<0.01)。不同浓度As2O3作用HUVEC48 h后检测上清液中ICAM-1与VCAM-1浓度时发现1μmol.L-1时VCAM-1表达即开始增加(123.32±3.78 mmol.L-1,P<0.01),而HUVEC表达ICAM-1含量与对照组相比差异并不明显(38.94±2.59 mmol.L-1,P>0.05),随着As2O3浓度的增加,HUVEC表达ICAM-1/VCAM-1的量均增加但敏感性不同。对照组及(1.0、2.0、3.0、4.0、5.0)μmol.L-1As2O3作用于HUVEC 48 h实时荧光定量PCR法检测VCAM-1mRNA表达量明显增加,与对照组相比实验组的表达量分别为(1.657±0.287,1.858±0.241,2.321±0.280,3.012±0.235,3.508±0.342)(P<0.01)。结论:As2O3可直接降低细胞活性,诱导细胞凋亡,并且呈一定的时间-浓度依赖性。在较低浓度时VCAM-1/ICAM-1的表达在一个相对较低的水平,随着As2O3浓度的逐渐升高,内皮细胞凋亡率增高,VCAM-1/ICAM-1表达增加,并且VCAM-1/ICAM-1对As2O3的敏感性呈现一定的差异性。

Influences of Arsenic Trioxide on Proliferation/Apoptosis and Expression of ICAM-1/VCAM-1 of Human Umbilical Vein Endothelial Cells

Objective: To investigate the effects of arsenic trioxide(As2O3) on the proliferation and apoptosis of endothelial cells and the influences of As2O3 on the expression of intercellular adhesion molecule-1(ICAM-1),vascular cell adhesion molecule-1(VCAM-1) in vitro.Methods: Human umbilical vein endothelial cells(HUVEC) were incubated in medium with different concentration of As2O3.The proliferation profile of HUVEC was determined by CCK8 Kit.The apoptosis of HUVEC was detected by microscopy and flow cytometry(FCM).The expression of ICAM-1/VCAM-1 was detected by applying ELISA and Real-time PCR.Results: The apoptosis rate of HUVEC was in a low level valued(0.134±0.03)%,(3.305±0.53)%,(3.748±0.84)%(P<0.05)when incubated in medium with 3 μmol·L-1 of As2O3 for 24 h,48 h and 72 h.When the concentration of As2O3 was more than 3 μmol·L-1,the apoptosis rate of HUVEC promoted significantly(P<0.01).The outcomes of ELISA showed that,compared with that in control group,the expression of VCAM-1 increased significantly(valued 123.32±3.78 mmol·L-1,P<0.01),while the expression of ICAM-1 did not change(P>0.05),when incubated in medium with 1.0 μmol·L-1 of As2O3.With the increasing of the concentration of As2O3,the reaction of ICAM-1/VCAM-1 to As2O3 was different.The expression of VCAM-1 mRNA(fold of change) were(1.657±0.287,1.858±0.241,2.321±0.280,3.012±0.235,3.508±0.342)(P<0.01) in 1.0,2.0,3.0 4.0 5.0 μmol·L-1 groups,respectively.Conclusion: Arsenic trioxide can promote the apoptosis of HUVEC,increase the expression of VCAM-1/ICAM-1 in a dosage and time dependent manner.