青藤碱抑制人宫颈癌的研究

目的：研究青藤碱（sinomenine，SIN）对宫颈癌Hela细胞增殖的影响及其机制，为SIN在宫颈癌的预防和治疗上提供实验依据。方法：不同浓度SIN分别处理体外培养的人宫颈癌细胞系Hela细胞后，采用噻唑蓝（Mar）法检测处理24h、48h、72h后Hela细胞的增殖活性，流式细胞仪测定细胞周期和细胞凋亡。结果：1．0．1、0．2、0．4、0．625、1．25、2．5mmml／L SIN处理Hela细胞24h、48h、72h后，细胞增殖明显受到抑制，呈时间和剂量依赖性特点；2．流式细胞仪细胞周期分析表明，SIN处理组G1期细胞比例明显增加，S期细胞比例明显减少，两组比较有统计学意义；3．细胞凋亡分析表明，SIN处理组细胞凋亡率较对照组升高，呈时间和剂量依赖性特点；结论：SIN在体外能有效抑制宫颈癌细胞生长，其机制可能与其阻滞细胞周期、诱导细胞凋亡有关，SIN有望应用于宫颈癌的辅助治疗。

Experimental study on the effect of selective COX- 2 inhibitor sinomenine on Hela cells

Objective: To study the inhibitory effect of a selective cyclooxygenase-2 inhibitor Sinomenine(SIN on Hela cells and further investigate the mechanism by which Sinomenine inhibited proliferation of Hela cells,and to afford laboratory data for application of SIN in the prevention and treatment for cervical cancer.Methods: Hela cells were treated with various concentration of SIN for 24,48 and 72h,the cell proliferation was estimated using the MTT assay,and the cell cycle distribution and apoptosis were examined by Flow cytometry.Results: SIN inhibits proliferation of Hela cells in a dose and time-dependent manner.Flow cytometry results demonstrated that the cell cycle was redistributed: the G1-Phase cell fraction was increased,while the S-Phase cell fraction was significantly decreased after the cells were treated with SIN(p<0.05);SIN could induce apoptosis of Hela cells in a dose and time-dependent manner.Conclusion: SIN could inhibit the growth of Hela cells,the mechanism may relate to its blockage of cell cycle and apoptosis induction.