| 游离脂肪酸对3T3-L1脂肪细胞糖代谢的影响 |
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| 引用本文： | 陆环,张君,徐文静,卢慧,吴江东,米利古,谢建新.游离脂肪酸对3T3-L1脂肪细胞糖代谢的影响[J].现代生物医学进展,2010,10(2). |
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| 作者姓名： | 陆环 张君 徐文静 卢慧 吴江东 米利古 谢建新 |
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| 作者单位： | 石河子大学医学院新疆地方病与民族高发病省部共建重点实验室,新疆,石河子,832000 |
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| 基金项目： | 973计划前期研究专项课题(2009CB526505) |
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| 摘 要： | 目的:通过培养3T3-L1前脂肪细胞,并诱导其分化至成熟,研究游离脂肪酸对脂肪细胞糖代谢的影响。方法:培养诱导3T3-L1脂肪细胞,用油红O染色鉴定并比较其形态结构的变化。LPS、EPA、SA、PA干预成熟脂肪细胞,收集不同时间的培养基,葡萄糖氧化酶法算出各组脂肪细胞的葡萄糖消耗量。用Western blot检测不同时间各组干预后细胞AMPK、GLUT4蛋白含量。结果:油红O染色鉴定成熟脂肪细胞胞浆中的脂滴染成红色,并出现戒环样结构;诱导分化第8天,90%以上细胞均分化成熟。含LPS、EPA、SA、PA的培养基作用于成熟脂肪细胞,随着时间的延长,显著抑制脂肪细胞对葡萄糖的吸收(P<0.05),同时,脂肪细胞AMPK、GLUT4蛋白含量在减少(P<0.05)。结论:游离脂肪酸可以诱导胰岛素抵抗的分子机制可能是通过胰岛素信号通路激活蛋白激酶(AMPK),进而影响GLUT4的蛋白表达,使脂肪细胞的葡萄糖吸收率减低,影响脂肪细胞的糖代谢。
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| 关 键 词： | 游离脂肪酸 3T3-L1脂肪细胞 葡萄糖消耗 GLUT4 |
Effects of free fatty acid on glucose metabolism in 3T3-L1 adipocytes |
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| LU Huan,ZHANG Jun,XU Wen-jing,LU Hui,WU Jiang-dong,MI Li-gu,XIE Jian-xin.Effects of free fatty acid on glucose metabolism in 3T3-L1 adipocytes[J].Progress in Modern Biomedicine,2010,10(2). |
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| Authors: | LU Huan ZHANG Jun XU Wen-jing LU Hui WU Jiang-dong MI Li-gu XIE Jian-xin |
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| Institution: | LU Huan,ZHANG Jun,XU Wen-jing,LU Hui,WU Jiang-dong,MI Li-gu,XIE Jian-xin(The KeyLaboratoryofXinJiangEndemic ,Ethnic Disease ofmedicalcollege ofShiheziUniversity,XinjiangShihezi832000,China) |
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| Abstract: | Objective:3T3-L1 cells were induced into adipocytes,to study the effects of free fatty acid on glucose metabolism in 3T3-L1 adipocytes. Methods:3T3-L1 cells were induced into adipocytes identified by Oil Red O staining,observe the morphologic transformation of 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated for LPS,EPA,SA,PA,collect adipocyte culture medium at different time point,Glucose Oxidase method was employed to measure the glucose consumption in the medium. Western blot was used to determine the p... |
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| Keywords: | FFA 3T3-L1 adipocytes glucose consumption GLUT4 |
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