康莱特联合顺铂对宫颈癌SiHa细胞增殖和凋亡的影响

目的:通过康莱特联合顺铂对宫颈癌SiHa细胞增殖和凋亡的影响,探讨其作用机制。方法:体外培养宫颈癌Siha细胞,分别将康莱特(浓度为1,2,4,6,8 mg/mL),顺铂(浓度梯度为1.5,3,6,9,12μg/mL),单独作用于宫颈癌SiHa细胞,加药24h、48h用噻唑蓝(MTT法)检测细胞增殖情况。用流式细胞术检测康莱特组和顺铂组细胞24h凋亡率,选取合适的药物浓度(康莱特6 mg/mL,顺铂3μg/mL),进行联合用药,加药24h、48h用MTT法检测细胞增殖情况,用流式细胞术检测24h细胞凋亡率。结果:①MTT法显示加药后两组的24h、48h,宫颈癌SiHa细胞的抑制率均高于对照组(P0.05),并且在一定程度上呈浓度和时间依赖性。②联合用药时,细胞的抑制率和凋亡率要显著高于单独用药(P0.01)。结论:康莱特、顺铂单独或联合作用均能抑制SiHa细胞的增殖,促进其凋亡,且康莱特联合顺铂的作用要显著高于单独用药,康莱特与化疗药物联合使用可提高肿瘤细胞对化疗药物的敏感性。

Effect of KLT combined with DDP on the Proliferation and Apoptosis of SiHa cell*

Objective： To investigate the effect of KLT combined with DDP on the proliferation and apoptosis of SiHa cell. Methods： Different concentrations of KLT injection （1, 2, 4, 6, 8 mg/ml）and DDP（1.5, 3, 6, 9, 12 μg/mL）were solo applied to SiHa cells. Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. The appropriate drug concentration （KLT 6 mg/ml,DDP 3 μg/mL）were selected. Then, KLT combined with DDP injection was applied to SiHa cells. Cell proliferation was measured by MTT, cell apoptosis was detected by flow cytometry. Results： ①Cell proliferation were dramatically inhibited by KLT injection （25,50,100,150,200 μg/mL）and DDP （1.5, 3, 6, 9, 12 μg/mL）at 24 h,48 h （P〈0.05）. In addition, Cell proliferation were dramatically inhibited by injected KLT combined with DDP. ②Combination therapy, apoptosis rate and inhibition rate of proliferation in SiHa cells treated by KLT combined with DDP were higher than the monotherapy. Conclusion： DDP, KLT and DDP combined with KLT can inhibit the proliferation, accelerate the apoptosis in SiHa cells. What＇s more, DDP combined with KLT have synergistic effect on the apoptosis in SiHa cells.