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| 转染Netrin-1基因的真皮多能干细胞治疗大鼠脊髓损伤的研究 | |
| 引用本文： | 陈景祥,王国华,任小宝,王涛,孙溦,屈纪富.转染Netrin-1基因的真皮多能干细胞治疗大鼠脊髓损伤的研究[J].现代生物医学进展,2010,10(3). |
| 作者姓名： | 陈景祥 王国华 任小宝 王涛 孙溦 屈纪富 |
| 作者单位： | 1. 第三军医大学西南医院急诊科,重庆,400038 2. 解放军第154医院内三科,河南,信阳,464250 3. 第三军医大学西南医院重症医学科,重庆,400038 |
| 基金项目： | 重庆市自然科学基金计划项目(CSTC,2007BB5040) |
| 摘 要： | 目的:观察转染Netrin-1基因的真皮多能干细胞(dMSCs)移植对大鼠脊髓损伤的修复作用。方法:取大鼠真皮组织,分离培养真皮多能干细胞,经转染Netrin-1基因和诱导,观察细胞形态变化,免疫细胞化学方法对分化细胞进行鉴定。Wistar大鼠在L4水平制成脊髓全横断损伤模型,伤处移植大鼠真皮多能干细胞或者转染Netrin-1基因的真皮多能干细胞。对大鼠进行动物行为学(BBB)评分和对损伤脊髓进行组织学检测。结果:转染Netrin-1基因的dMSCs诱导产生的神经元样细胞占总细胞数的比例为24.45±3.73%,而单独的真皮多能干细胞诱导产生的神经元样细胞占总细胞数的比例10.50±2.13%,二者差异显著(P<0.05)。BBB评分显示转染Netrin-1基因的dMSCs移植组明显高于单纯dMSCs移植组和空白对照组(P<0.05);转染Netrin-1基因的dMSCs移植组损伤脊髓结构的修复明显优于单纯dMSCs移植组和空白对照组。结论:转染Netrin-1基因的真皮多能干细胞移植较单纯dMSCs移植对大鼠脊髓损伤有更好的治疗作用。 |
| 关 键 词： | Netrin-1 真皮多能干细胞 脊髓损伤 |
Study on treatment of spinal cord injured in rats by implantation of dermal multipotent stem cells transfected with Netrin-1 gene |
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| CHEN Jing-xiang WANG Guo-hua REN Xiao-bao WANG Tao SUN Wei,QU Ji-fu.Study on treatment of spinal cord injured in rats by implantation of dermal multipotent stem cells transfected with Netrin-1 gene[J].Progress in Modern Biomedicine,2010,10(3). | |
| Authors: | CHEN Jing-xiang WANG Guo-hua REN Xiao-bao WANG Tao SUN Wei QU Ji-fu |
| Institution: | CHEN Jing-xiang1 WANG Guo-hua1 REN Xiao-bao1 WANG Tao2 SUN Wei1,QU Ji-fu1 (Emergency department,Southwest Hospiital,Third Military Medical University,Chongqing 400038,China,Department of Medicine,No.154 Hospital of PLA,Xinyang464250,Intensive care unit of Southwest Hospiital,China) |
| Abstract: | Objective:To observe the treatment of spinal cord injury in rats with transplantation of dermal multipotent stem cells (dMSCs) transfected with Netrin-1 gene. Methods:Dermis of neonatal rat was isolated and the dMSCs were cultured, which were transfected with Netrin-1 gene. The differentiated cells were identified by immunohistochemical technique. The spinal cord injury of adult Wistar rats was performed in L4. dMSCs transfected with or without Netrin-1 gene were injected into the injured spinal cord. The b... |
| Keywords: | Netrin-1 dermal multipotent stem cell spinal cord injury |
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