复方醋酸棉酚片联合独一味胶囊对大鼠无排卵型功能失调性子宫出血的改善作用及其作用机制

摘要 目的：探讨复方醋酸棉酚片联合独一味胶囊对大鼠无排卵型功能失调性子宫出血的改善作用及其作用机制。方法：将40只雌性SD大鼠随机均分成两组（空白组和模型组），采用给大鼠连续21 d灌胃戊酸雌二醇的方法制备无排卵型功能失调性子宫大鼠模型。模型大鼠制备成功后，按随机法再均分成模型组、阳性组和治疗组，其中阳性组和治疗组分别灌胃给予相应的药物，空白组和模型组灌胃等体积的生理盐水，各组均连续灌胃7 d。计算各组大鼠的子宫系数，采用酶联免疫吸附法测定大鼠血清中雌二醇（estradiol, E₂）和孕酮（progestone，P）水平，采用免疫组化法测定大鼠子宫内膜组织中两个蛋白（matrix metalloproteinases-9，MMP-9和vascular endothelial growth factor ,VEGF）的表达量。结果：模型组大鼠血清中的P水平比空白组显著性减少（P<0.05），子宫内膜组织中的MMP-9和VEGF表达量比空白组均显著性升高（P<0.05）。给药后，治疗组和阳性组大鼠血清中的P水平均显著高于模型组（P<0.05），大鼠子宫内膜组织中的MMP-9和VEGF 表达量均显著低于模型组（P<0.05），治疗组与阳性组之间无显著性差异（P >0.05）。结论：复方醋酸棉酚片联合独一味胶囊对无排卵型功能失调性子宫出血具有明显的改善作用，其机制可能与调控子宫内膜 MMP-9 和VEGF的表达量有关。

Improvement of Compound Gossyl Acetate Tablets Combined with Duyiwei Capsules on Anovulatory Dysfunctional Uterine Bleeding Induced by Estradiol Valerate in Rats

ABSTRACT Objective: To investigate the improvement and mechanism of Compound gossyl acetate tablets combined with Duyiwei capsules on anovulatory dysfunctional uterine bleeding induced by estradiol valerate in rats. Methods: A total of 40 female SD rats were randomly divided into two groups (blank group and model group). The anovulatory dysfunctional uterine model was prepared by giving rats estradiol valerate by gavage for 21 consecutive days. After the model rats were successfully prepared, they were divided into model group, positive group and treatment group according to the random method, in which the positive group and treatment group were given corresponding drugs by gavage respectively, and the blank group and model group were given the same volume of normal saline by gavage, and each group was given continuous gavage for 7 days. The uterine coefficients of each group were calculated. The serum estradiol (E₂) and progesterone (P) levels were measured by enzyme-linked immunosorbent assay (ELISA), and the expression levels of matrix Metalloproteinases-9 (MMP-9) and vascular growth factor (VEGF) in the endometrial tissues were measured by immunohistochemistry. Results: The serum Level of P in the model group was significantly lower than that in the blank group (P<0.05), and the expression levels of MMP-9 and VEGF in the endometrial tissues were significantly higher than that in the blank group (P<0.05). After administration, the serum P level of rats in both the treatment group and the positive group was significantly higher than that in the model group (P<0.05), and the expression levels of MMP-9 and VEGF in the endometrial tissues of rats were significantly lower than that in the model group (P<0.05). There was no significant difference between the treatment group and the positive group (P>0.05). Conclusion: Compound gossyphenol acetate tablet combined with Duyiwei capsule can significantly improve anovulatory dysfunction uterine bleeding, and the mechanism may be related to the regulation of the expression levels of MMP-9 and VEGF in endometrium.