超声重复辐照孕鼠对子代海马NMDA神经损伤的影响

摘要 目的：探讨超声重复辐照孕鼠对子代海马N-甲基-D天冬氨酸(N-methyl-D aspartic acid，NMDA)神经损伤的影响。方法：孕12~14 d昆明种小鼠27只随机平分为三组-对照组、短时间辐照组、长时间辐照组。用超声探头在各组孕鼠进行辐照0 min、10 min与20 min，让母鼠自然分娩哺育幼仔，将各组仔鼠随机挑选12只，检测仔鼠海马组织NMDA表达与神经损伤情况。结果：辐照过程中无孕鼠死亡，短时间辐照组、长时间辐照组仔鼠第30 d与60 d的总路程、中央路程、中央时间都少于对照组(P<0.05)，长时间辐照组低于短时间辐照组(P<0.05)。短时间辐照组、长时间辐照组仔鼠第60 d的神经元细胞凋亡指数、海马组织乙酰胆碱酯酶含量都高于对照组(P<0.05)，NMDA蛋白相对表达水平低于对照组(P<0.05)，长时间辐照组与短时间辐照组对比差异也都有统计学意义(P<0.05)。结论：超声重复辐照孕鼠能抑制仔鼠海马组织NMDA蛋白表达，促进神经元细胞凋亡与提高乙酰胆碱酯酶含量，从而降低仔鼠的自主记忆活动能力。

Effects of Repeated Ultrasound Irradiation of Pregnant Rats on the Hippocampal NMDA Nerve Injury of Offspring

ABSTRACT Objective: To investigate the effects of repeated ultrasound irradiation of pregnant rats on the offspring''s hippocampal N-methyl-D aspartic acid (NMDA) nerve damage. Methods: 27 cases of Kunming mice of 12-14 days gestation were randomly equally divided into three groups-control group, short-term irradiation group and long-term irradiation group. The cases in each group were irradiated with Repeated Ultrasound Irradiation for 0 min, 10 min and 20 min. The mother mice were allowed to give birth to their pups naturally, the offspring were randomly reduced by 12 cases in each groups, and were to detect the expression of NMDA and nerve damage in the hippocampus of the offspring. Results: There were no pregnant mice died during the irradiation. The total distance, central distance and central time of the offspring on the 30 th and 60 th day of the short-term and long-term radiation groups were less than those of the control group (P<0.05), the irradiation group were lower than the short-term irradiation group(P<0.05). The neuronal apoptosis index and hippocampal acetylcholinesterase content of the short-term irradiation group and the long-term irradiation group on the 60 th day were higher than those of the control group(P<0.05), and the relative expression level of NMDA protein were lower than that of the control group(P<0.05), the difference compared between the long-term irradiation group and the short-term irradiation group were also statistically significant(P<0.05). Conclusion: Repeated ultrasound irradiation of pregnant mice can inhibit the expression of NMDA protein in hippocampus of offspring, promote neuronal cell apoptosis and increase the content of acetylcholinesterase, thereby reduce the ability of autonomous memory of offspring.