阿霉素注射剂量对肾病综合征大鼠脂蛋白脂酶和卵磷脂胆固醇酰基转移酶水平的影响

摘要 目的：探讨阿霉素注射剂量对肾病综合征（nehpmtic syndrome，NS）大鼠脂蛋白脂酶和卵磷脂胆固醇酰基转移酶（Leci-thin cholesterol acyltransferase，LCAT）水平的影响。方法：64只SD大鼠随机平分为四组-对照组、小剂量阿霉素组、中剂量阿霉素组与高剂量阿霉素组，四组大鼠经尾静脉一次性注射阿霉素0 mg/kg、2 mg/kg、4 mg/kg、8 mg/kg，检测造模后不同时间点大鼠肾脏脂蛋白脂酶和卵磷脂胆固醇酰基转移酶水平变化情况。结果：小剂量阿霉素组、中剂量阿霉素组与高剂量阿霉素组造模后7 d、14 d、21 d的体重与每日采食量、血肌酐与尿素氮都低于对照组（P<0.05），24 h尿蛋白高于对照组（P<0.05），且存在剂量依赖性，三组间对比差异有统计学意义（P<0.05）。小剂量阿霉素组、中剂量阿霉素组与高剂量阿霉素组造模后21 d、28 d的肾脏脂蛋白脂酶和卵磷脂胆固醇酰基转移酶相对表达水平低于对照组（P<0.05），且存在剂量依赖性，三组间对比差异有统计学意义（P<0.05）。结论：小剂量阿霉素可抑制大鼠肾脏脂蛋白脂酶和卵磷脂胆固醇酰基转移酶的表达，能快速有效建立肾病综合征大鼠模型，具有很好的模拟造模效果。

Effect of Doxorubicin Injection Dose on the Levels of Lipoprotein Lipase and Lecithin Cholesterol Acyltransferase in Nephrotic Syndrome Rats

ABSTRACT Objective: To investigate the effects of doxorubicin injection on the levels of lipoprotein lipase and leci-thin cholesterol acyltransferase (LCAT) in rats with nehpmtic syndrome (NS). Methods: Sixty-four SD rats were randomly divided into four groups, namely: matched group, low-dose adriamycin group, medium-dose adriamycin group and high-dose adriamycin group. The four groups of rats were injected with adriamycin through the tail vein at one time at 0 mg/kg, 2 mg/kg, 4 mg/kg, 8 mg/kg, the changes in rat kidney lipoprotein lipase and LCAT levels were detected at different time points after modeling. Results: The body weight, daily feed intake, blood creatinine and urea nitrogen of the low-dose doxorubicin group, medium-dose doxorubicin group and high-dose doxorubicin group were lower than those of the matched group (P<0.05) at 7 d, 14 d, 21 d after modeling, the 24 h urine protein were higher than that of the matched group (P<0.05), and there were adose-dependence, the difference compared among the three groups were statistically significant (P<0.05). The relative expression levels of renal lipoprotein lipase and lecithin cholesterol acylase in the low-dose doxorubicin group, medium-dose doxorubicin group, and high-dose doxorubicin group were lower than those in the matched group at 21 d and 28 d after modeling (P<0.05). and there were dose-dependence, the difference compared among the three groups were statistically significant(P<0.05). Conclusion: A small dose of doxorubicin can inhibit the expression of lipoprotein lipase and LCAT in rat kidneys, and can quickly and effectively establish rat model of nephrotic syndrome with good simulation effect.