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| 左侧大肠癌和右侧大肠癌蛋白质差异表达谱的建立及质谱分析 | |
| 引用本文： | 曾亮,裴海平,朱红,吴胜其,汤明,黄林生,陈森林.左侧大肠癌和右侧大肠癌蛋白质差异表达谱的建立及质谱分析[J].现代生物医学进展,2010,10(24). |
| 作者姓名： | 曾亮 裴海平 朱红 吴胜其 汤明 黄林生 陈森林 |
| 作者单位： | 1. 湖南省肿瘤医院病理科,湖南,长沙,410013 2. 中南大学湘雅医院普外科,湖南,长沙,410008 3. 中南大学湘雅医院肿瘤科,湖南,长沙,410008 4. 湖南省肿瘤医院中心实验室,湖南,长沙,410013 5. 湖南省肿瘤医院腹外二科,湖南,长沙,410013 |
| 基金项目： | 湖南省卫生厅科研基金课题(C2009-010); 国家自然基金项目(30872463) |
| 摘 要： | 目的:初步探索左侧大肠癌和右侧大肠癌中蛋白质表达的差异,为左右侧大肠癌生物学特性上的差别提供功能基因组学上的依据。方法:以左右侧大肠癌组织为研究对象,提取组织总蛋白,依次进行二维凝胶电泳,凝胶图象分析,质谱及生物信息学分析。结果:成功建立了左侧大肠癌和右侧大肠癌的二维电泳图谱,进行质谱和生物信息学分析比较得到左侧大肠癌与右侧大肠癌的差异表达蛋白共有16个,其中左侧大肠癌中表达增加的蛋白有10个包括蛋白质二硫化异构酶A1,78 kDa葡萄糖调节蛋白,抑制素,热休克蛋白60,含硫氧还蛋白域的蛋白5,T-复合蛋白1ε亚单位,应急蛋白70,异柠檬酸脱氢酶,蛋白质二硫化异构酶A3,巨噬细胞加帽蛋白;左侧大肠癌表达降低的蛋白6个包括ATP合成酶β亚单位,延伸因子1-delta,热休克蛋白β1,载脂蛋白A-Ⅰ,转甲状腺素蛋白,热休克蛋白β6。结论:左侧大肠癌和右侧大肠癌中存在差异表达蛋白,这些差异表达蛋白可能是左右侧大肠癌生物学性质差异的分子遗传学基础。 |
| 关 键 词： | 左侧大肠癌 右侧大肠癌 二维电泳 质谱 |
Establishment and Mass Chromatographic Analysis of Differentially Expressed Protein Spectrum between left-sided and right-sided large intestine carcinoma |
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| ZENG Liang,PEI Hai-ping,ZHU Hong,WU Sheng-qi,Tang Ming,HUANG Lin-sheng,CHEN Sen-lin.Establishment and Mass Chromatographic Analysis of Differentially Expressed Protein Spectrum between left-sided and right-sided large intestine carcinoma[J].Progress in Modern Biomedicine,2010,10(24). | |
| Authors: | ZENG Liang PEI Hai-ping ZHU Hong WU Sheng-qi Tang Ming HUANG Lin-sheng CHEN Sen-lin |
| Institution: | ZENG Liang1,PEI Hai-ping2,ZHU Hong3,WU Sheng-qi4,Tang Ming5,HUANG Lin-sheng2,CHEN Sen-lin1(1 Department of pathology,Hunan Tumor Hospital Changsha 410013 P.R.China,P.R.China,2 Department of general Surgery,3 Department of Clinical Oncology,Xiangya Hospital of Central-south University Key Changsha 410008,4 Medicine Laboratory,5 Department of pathology,Hunan Tumor Hospital Changsha 410013 P.R.China) |
| Abstract: | Objective: To investigate differentially expressed proteins between left-sided large intestine carcinoma and right-sided large intestine carcinoma,molecular genetic basis in difference of of biological characters between left-sided large intestine carcinoma and right-sided large intestine carcinoma was provided.Methods: Total tissue protein of left-sided large intestine carcinoma and right-sid-ed large intestine carcinoma were extracted,differential proteome profiles were established and analysized by means... |
| Keywords: | left-sided large intestine carcinoma right-sided large intestine carcinoma two-dimensional electrophoresis mass spectrometry |
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