Construction and characterization of a bacterial artificial chromosome library of maize inbred line 77Ht2 |
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Authors: | Jiliang?Yang Qinghua?Wang Daiyong?Deng Manli?Weng Xiaoyan?Yin Demin?Jin Juren?Zhang Email author" target="_blank">Bin?WangEmail author |
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Institution: | (1) Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China;(2) School of Life Science, Shandong University, 250100 Jinan, China |
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Abstract: | Maize inbred line 77Ht2 contains agriculturally important genes and has been widely used in corn breeding in China. A bacterial
artificial chromosome (BAC) library of 77Ht2 has been constructed in order to identify useful genes and to facilitate the
study of the maize genome. The library contains 175104 clones with an average insert size of 57 kb and represents about 4
maize haploid genome equivalents. Characterization of the library showed less than 0.5% of clones to not contain large inserts.
Significant contamination of chloroplast and mitochondria DNA was not detected. BAC clones (152 arrays) were stored in 96
microtiter plates, with each well containing 12 clones. This is the first maize BAC library constructed in China. It is well
suited for map-based cloning of maize genes and genome physical mapping. |
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Keywords: | 77Ht2 bacterial artificial chromosome maize |
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