Microextraction of Nuclear Proteins from Single Maize Embryos |
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| Authors: | Busk Peter K Pagès Montserrat |
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| Institution: | (1) Departament de Genètica Molecular, Centre d'Investigació i Desenvolupament, C.S.I.C. Jordi Girona 18-26, 08034 Barcelona, Spain |
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| Abstract: | The analysis of DNA binding proteins can be difficult when only small quantities of tissue expressing the desired protein are available. We present a protocol for the preparation of nuclear extracts from as little as 100 mg of tissue. This protocol is well suited for extraction of DNA binding proteins from tissues that are difficult to obtain in large quantities such as maize embryos. |
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| Keywords: | band shift assay DNA binding protein maize nuclear protein extraction |
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