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RAPD markers in diversity detection and variety identification of Tibetan hulless barley
Authors:Email author" target="_blank">Zeng?YuEmail author  Lan?Li-Qiong  Luo?Huan  Bai?Jie  Yang?Man-Ye  Miao?Chen  Cai?Ying-Fan  Qiang?Xiao-Lin  Chen?Fang
Institution:(1) College of Life Science, Sichuan University, 610064 Chengdu;(2) College of Life Science, Peking University, 100871 Beijing;(3) Tibet Agriculture Research Institute, 850000 Tibet, China
Abstract:RAPD markers were generated from 6 groups of Tibetan hulless barley (23 varieties): Lasagoumang, QB, Zangqing, Guoluo, Dongqing, and Hymalayia. Of the 48 fragments generated by 5 selected primers (among 68 primers), 44 appeared to be polymorphic (92%). Cluster analysis was performed (RAPDistance 1.04). The 23 varieties were divided into 2 groups, and the molecular foundation of genetic diversity was explored. In addition, to identify the varieties, one DNA fingerprint was constructed based on 17 bands amplified with S32 and 2 bands with S18. A specific RAPD fragment can be used to select for high and low β-glucan content varieties. Supported by the important program of Ministry of Science and Technology of P.R. China, “The Construction of Key Animal & Plant Resource System of Southwest China”.
Keywords:cluster analysis  genetic diversity  β  -glucan  RAPD  Tibetan hulless barley
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