A simple method forIn Situ hybridization to RNA in guard cells ofVicia Faba L.: The expression of aquaporins in guard cells |
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| Authors: | Mei-Hao Sun Wen Xu Ya-Fang Zhu Wei-Ai Su Zhang-Cheng Tang |
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| Institution: | (1) Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences/CAS, P. R. China;(2) Department of Mathematics, University of Louisville, 40292 Louisville, KY |
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| Abstract: | Because the epidermis ofV. faba L. leaves easily can be peeled into strips of one cell layer, we developed a simple method ofin situ hybridization using epidermal peels as a substitute for paraffin, resin and cryosections. Our method sufficiently detected
the expression of broad bean aquaporin 1 in guard cells. RT-PCR revealed higher expression of aquaporins (AQPs) in guard cells
compared to other leaf cell types; this indicates the importance of AQP for bulk water flow across guard cell membranes and,
therefore, for stomatal movements. |
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| Keywords: | aquaporin epidermis guard cell in situ hybridization Vicia faba |
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