Isolation of plant DNA: A fast,inexpensive, and reliable method |
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Authors: | Pierre Guillemaut Laurence Maréchal-Drouard |
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Institution: | (1) Laboratoire de Biologie Végétale Appliquée, Institut Universitaire de Technologie Louis Pasteur, 3 rue de l’Argonne, F-67000 Strasbourg, France;(2) Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, F-67084, Strasbourg Cedex, France |
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Abstract: | We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees,
herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction
is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with
highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or
detection of adducts. |
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Keywords: | DNA isolation woody plant seed cell culture |
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