A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis |
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| Authors: | Andrew H Paterson Curt L Brubaker Jonathan F Wendel |
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| Institution: | (1) Present address: Department of Soil and Crop Sciences, Texas A & M University, 77843-2474 College Station, TX, USA;(2) Department of Botany, Iowa State University, 50011 Ames, IA, USA |
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| Abstract: | Extraction of high-quality genomic DNA fromGossypium (cotton) species is difficult due to high levels of polysaccharide, oxidizable quinones, and other interfering substances.
We describe a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP
and PCR analysis, as well as for most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol
oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation
of nuclei. |
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| Keywords: | cotton genomic DNA extraction Gossypium PCR RFLP |
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