Isolation and Characterization of a Curcin Promoter from <Emphasis Type="Italic">Jatropha curcas</Emphasis> L. and Its Regulation of Gene Expression in Transgenic Tobacco Plants |
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Authors: | Xiaobo Qin Jinping Zhang Caixia Shao Sha Lin Luding Jiang Shuwen Zhang Ying Xu Fang Chen |
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Institution: | (1) College of Life Sciences, Sichuan University, Chengdu, 610064, People’s Republic of China |
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Abstract: | Ribosome-inactivating proteins (RIPs) represent those proteins that universally depurinate conserved α-sarcin loops of large
rRNAs. In this study, a 0.6-kb fragment of a 5′ flanking region preceding a curcin gene, encoding a type I RIP curcin, of
Jatropha curcas L. endosperm was cloned, and its regulation of expression of the β-glucuronidase (GUS) reporter gene was investigated in
transgenic tobacco. Analysis of GUS activities showed that the 0.6-kb flanking fragment of the curcin gene was sufficient
to drive the GUS reporter gene expression in tobacco seed. The activity of this flanking fragment was analyzed at different
stages of seed development. Histochemical localization of GUS activity indicated that the promoter was specifically active
in the endosperm tissue of the dicotyledonous tobacco embryo. Moreover, this activity was first initiated at the heart-shaped
embryonic stage during seed development. |
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Keywords: | Curcin Promoter Jatropha curcas L Ribosome-inactivating protein |
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