Elimination of oxalate contamination in RNA isolation from<Emphasis Type="Italic">Rumex obtusifolius</Emphasis> |
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Authors: | Email author" target="_blank">Glenda?W?TinneyEmail author Susan?C?Pritchard Raquel?Gonzalez Nigel?D?Paul Paul?E?Hatcher Jane?E?Taylor |
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Institution: | (1) Department of Biological Sciences, Institute of Environmental and Natural Sciences, LA1 4YQ Lancaster, UK;(2) Department of Agricultural Botany, School of Plant Sciences, University of Reading, 2, Earley Gate, Whiteknights, RG6 6AU Reading, UK |
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Abstract: | Many plant RNA isolation techniques aim to prevent contamination by means of secondary phenolics, carbohydrates, RNase, and
other chemicals. However, when applied in our laboratory to the isolation of RNA fromRumex obtusifolius, these protocols failed to produce good quality RNA. A major problem was contamination of the RNA samples with the secondary
metabolite oxalate. The relative quantities of guanidine isothiocyanate extraction buffer to plant tissue used in the protocol
had significant effects on oxalate contamination. An increase in extraction buffer, from 1.5 mL in the original method to
15 mL per 200–300 mg of tissue in our protocol, removed the oxalate from the RNA. This RNA was of a good quality and was suitable
for molecular biology applications. |
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Keywords: | RNA extraction oxalate contamination Rumex |
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