DNA extraction from plants: The use of pectinase |
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Authors: | Steven H Rogstad Brian Keane Carolyn Howes Keiffer Fred Hebard Paul Sisco |
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Institution: | (1) Department of Biological Sciences ML6, University of Cincinnati, 45221-00006 Cincinnati, OH;(2) Department of Botany, Miami University, E. University Blvd., 4200 Middletown, OH, 45042;(3) American Chestnut Foundation, 14005 Glenbrook Ave., 24361 Meadowview, VA |
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Abstract: | Several earlier protocols for extracting plant DNA or RNA do not work well for a variety of plants because contaminating substances
coprecipitate with the nucleic acids and, thus, are present even at the last DNA-hydration step. While DNA extraction protocols
have been published in which pectinase is employed to break down these contaminating substances, here we present an alternative
modified pectinase protocol that potentially uses fewer steps and avoids the use of ethylene glycol monoethyl ether and phenol.
DNA analyses results are described for 6 plant species demonstrating that the method works across distantly related plant
taxa. |
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Keywords: | DNA contamination removal pectin degradation plant DNA extraction plant RNA extraction polygalacturonase |
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