A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications |
| |
Authors: | Chen D-H Ronald PC |
| |
Institution: | (1) Department of Plant Pathology, University of California, 1 Shields Ave., Davis , CA 95616, USA |
| |
Abstract: | A rapid DNA minipreparation method was developed for rice and other plant species. This method uses an Eppendorf tube and 1-ml pipette tip to grind plant tissues, and requires only one transfer for DNA isolation. In a single day, one person can complete DNA isolation from more than 120 leaf samples. The yields of the DNA samples ranged from 2.3 to 5.2 g from 25–50 mg fresh leaf tissue. DNA samples extracted using this method from rice were completely digested with five restriction enzymes (EcoR I, EcoR V, Hind III, Mse I and Pst I) and were successfully used for AFLP and other PCR applications. |
| |
Keywords: | CTAB DNA extraction |
本文献已被 SpringerLink 等数据库收录! |
|