Improved technique for isolating RNA from tobacco tissues |
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Authors: | Myeong-Hyeon Wang Hak-Ik Rhee |
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Institution: | (1) Division of Food and Biotechnology, Kangwon National University, Chuncheon, 200-701 Kangwon, Korea |
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Abstract: | We have developed a much-improved method for isolating RNA from tobacco tissue. The novel component of the described RNA isolation
method is the addition of lithium chloride to the extraction buffer. Following that, the RNA was homogenized with phenol/chloroform
and precipitated in ethanol. This isolation technique provided highly reproducible and good quality RNA within 2 h. |
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Keywords: | lithium chloride RNA tobacco tissue |
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