A method for the medium-term storage of plant tissue samples at room temperature and successive cycles of DNA extraction |
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| Authors: | Caroline Tassy Catherine Feuillet Pierre Barret |
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| Institution: | (1) Plateforme de Transgénèse du Blé, Université Blaise Pascal, 234, avenue du Brézet, 63100 Clermont-Ferrand, France;(2) Structure, Fonction, et évolution du Génome du Blé, UMR ASP 1095 INRA, Université Blaise Pascal, 234, avenue du Brézet, 63100 Clermont-Ferrand, France |
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| Abstract: | Based on the protocol originally described by Stein et al. (2001), we have developed a method that allows for medium-term
conservation at room temperature of wheat (Triticum aestivum) tissue samples to use for DNA extraction. DNA quality was suitable for analysis by PCR and Southern hybridization, even
after 2 months of storage at room temperature. This method allows successive DNA re-extractions from a previously extracted
sample and maximization of the DNA yield that can be recovered from precious samples. This method has applications for conservation
of leaf samples and management of DNA extraction. Our method can help improve data recovery in many plant molecular genetics
research projects. |
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| Keywords: | DNA extraction PCR sample conservation Southern blot wheat yield |
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