A simple procedure for the isolation of high quality RNA from ripening banana fruit |
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Authors: | Mehar H Asif Puneet Dhawan Pravendra Nath |
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Institution: | (1) Plant Gene Expression Laboratory, National Botanical Research Institute, Rana Pratap Marg, 226001 Lucknow, India |
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Abstract: | Isolation of high quality RNA from ripening banana fruit tissue is difficult due to high levels of polysaccharides and other
substances that interfere when using conventional procedures for RNA isolation. These substances not only decrease the yield
but the quality of RNA is almost unusable. We describe here a simple RNA procedure that effectively removes these contaminating
substances without affecting the yield. Following this procedure, we routinely obtained 80–150 μg of total RNA per g fresh
tissue. The RNA is of good quality and suitable for northern analysis, RT-PCR and cDNA library construction.
NBRI publication No. 488(NS). |
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Keywords: | Banana fruit tissue Musa acuminata polysaccharides RNA extraction |
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