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Development of microsatellite markers for Qualea grandiflora (Vochysiaceae), a typical species of the Brazilian cerrado
Authors:Ritter Lia Maris Orth  Bajay Miklos Maximiliano  Monteiro Mariza  Souza Renata G V C  Moreno Maria Andréia  Kageyama Paulo Yoshio
Institution:Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Av. Padua Dias 11, Piracicaba, São Paulo 13400-970, Brazil
Abstract:? Premise of the study: Microsatellite primers were developed to investigate genetic diversity and population structure of Qualea grandiflora, a typical species of the Brazilian cerrado. ? Methods and Results: Eight microsatellite loci were isolated using an enrichment cloning protocol. These loci were tested on a population of 110 individuals of Q. grandiflora collected from a cerrado fragment in S?o Paulo State, Brazil. The loci polymorphism ranges from seven to 19 alleles and the average heterozygosity value is 0.568, while the average polymorphic information content is 0.799. ? Conclusions: The developed markers were found to be highly polymorphic, indicating their applicability to studies of population genetic diversity in Q. grandiflora.
Keywords:cerrado  genetic diversity  microsatellites  Qualea grandiflora  simple sequence repeat markers  Vochysiaceae
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