Microsatellite markers for the relictual dove tree, Davidia involucrata (Cornaceae) |
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Authors: | Tao Chengcheng Yang Zhenyan Lu Ranran Liu Tao Chen Shaotian Ji Yunheng |
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Institution: | Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China. |
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Abstract: | ? Premise of the study: Microsatellite markers were developed for the dove tree, Davidia involucrata (Cornaceae), a Tertiary relict currently endemic to China, to investigate its population genetics and phylogeography. ? Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, nine polymorphic microsatellite loci were identified and screened in 44 individuals from three wild populations of D. involucrata. The number of alleles per locus ranged from two to three, while the observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.6000 and from 0.0000 to 0.6323, respectively. ? Conclusions: These new microsatellite loci will facilitate further studies of the population genetics and phylogeography of D. involucrata, as well as of the evolutionary history of the plant and other Tertiary relicts endemic to East Asia. |
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Keywords: | Cornaceae Davidia involucrata microsatellite polymorphic population genetics |
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