Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. |
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| Authors: | B Bartolomé Y Jubete E Martínez F de la Cruz |
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| Institution: | Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain. |
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| Abstract: | A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ alpha reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (CmR)-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KmR)-encoding gene and the same six MCS; and (iii) two CmR vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ alpha reporter gene of pUC18/19. |
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