The DNA between Rz and cosR in bacteriophage lambda is nonessential |
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| Authors: | V J Hernandez T D Edlind R F Young G M Ihler |
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| Institution: | 1. Department of Biology, University of Texas at Dallas P.O. Box 830688, Richardson, TX 75083-0688 U.S.A. Tel. (214) 690-2500;2. Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia, PA 19129, U.S.A. Tel. (215) 842-6908;3. Department of Medical Biochemistry and Genetics, Texas A&M University, College Station, TX 77843-1112 U.S.A. Tel. (409) 845-2726 |
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| Abstract: | Near the right end of phage lambda DNA, between gene Rz and the cos site, are 2050 bp of apparently non-coding DNA. We have cloned a lambda DNA fragment containing this DNA into a plasmid and constructed a deletion, omega l, extending from a site within the Rz gene to a site about 560 bp from cos. This deletion could be recombined into viable lambda phage at a frequency equal to that observed for the undeleted sequence. Recombinant phage lambda carrying the omega l deletion were demonstrated to have the same burst size and kinetics of phage production as undeleted lambda. The omega l deletion can be used to extend the capacity of lambda cloning vectors and to provide a region for the insertion of heterologous DNA which should exhibit controllable high level expression from the lambda late promoter, p'R. |
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| Keywords: | Recombinant DNA cloning vectors plasmids promoter high-level expression deletions b central region of λ genome (see Daniels et al 1983) bp base pairs λ cohesive end site kb 1000 bp pfu plaque-forming units ω To whom correspondence and reprint requests should be addressed |
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