Regulation and expression of the bacteriophage mu mom gene: mapping of the transactivation (dad) function to the C region |
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Authors: | S Hattman J Ives W Margolin M M Howe |
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Institution: | 1. Department of Biology, University of Rochester, Rochester, NY 14627, U.S.A. Tel. (716)275-3846;1. Department of Bacteriology, University of Wisconsin, Madison, WI 53706 U.S.A. Tel. (608)262-3052 |
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Abstract: | Expression of the bacteriophage Mu mom gene is under tight regulatory control. One of the factors required for mom gene expression is the trans-acting function (designated Dad) provided by another Mu gene. To facilitate studies on the signals mediating mom regulation, we have constructed a mom-lacZ fusion plasmid which synthesizes beta-galactosidase only when the Mu Dad transactivating function is provided. lambda pMu phages carrying different segments of the Mu genome have been assayed for their ability to transactivate beta-galactosidase expression by the fusion plasmid. The results of these analyses indicated that the Dad transactivation function is encoded between the leftmost EcoRI site and the lys gene of Mu; this region includes the C gene, which is required for expression of all Mu late genes. Cloning of an approx. 800-bp fragment containing the C gene produced a plasmid which could complement MuC- phages for growth and could transactivate the mom-lacZ fusion plasmid to produce beta-galactosidase. These results suggest that the C gene product mediates the Dad transactivation function. |
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Keywords: | Recombinant DNA gene fusion β-galactosidase phage vector λpMu Amp ampicillin bp base pair(s) Cam chloramphenicol 1CD intercistronic divide kb kilobase pairs moi multiplicity of infection resistance Tc tetracycline Tet tetracycline XGal [] indicates plasmid-carrier state :: novel joint Δ deletion |
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