A new revision of the sequence of plasmid pBR322 |
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Authors: | Ned Watson |
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Institution: | Molecular Biology Research, The Upjohn Company, Kalamazoo, MI 49007, U.S.A. |
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Abstract: | A revised sequence in the region immediately upstream from the rop gene of pBR322 is reported. Two base pairs in the accepted sequence do not exist in the plasmid DNA. Specifically, a TA base pair is missing at sequence coordinate 1893 Sutcliffe, Cold Spring Harbor Symp. Quant. Biol. 43 (1979) 77–90] and an AT base pair is missing at position 1915, giving a total size for pBR322 of 4361 bp. These changes are in a potential translation initiation sequence and probably reflect errors in the original sequence rather than recent evolution of the plasmid. |
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Keywords: | Plasmid rop gene expression vector translation initiation sequence correction conserved sequences pMBl |
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