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Contribution of genetic and epigenetic mechanisms to Wnt pathway activity in prevalent skeletal disorders
Authors:Carmen García-Ibarbia  Jesús Delgado-Calle  Iñigo Casafont  Javier Velasco  Jana Arozamena  María I Pérez-Núñez  María A Alonso  María T Berciano  Fernando Ortiz  José L Pérez-Castrillón  Agustín F Fernández  Mario F Fraga  María T Zarrabeitia  José A Riancho
Institution:1. Department of Internal Medicine, Hospital U.M. Valdecilla-IFIMAV, University of Cantabria, RETICEF, Santander, Spain;2. Department of Anatomy and Cell Biology, University of Cantabria, IFIMAV, Santander, Spain;3. Department of Orthopaedic Surgery and Traumatology, Hospital U.M. Valdecilla, Santander, Spain;4. Department of Internal Medicine, Hospital U, Rio-Hortega, University of Valladolid, Valladolid, Spain;5. Cancer Epigenetics Laboratory, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), HUCA, University of Oviedo, Oviedo, Spain;6. Unit of Legal Medicine, University of Cantabria, IFIMAV, Santander, Spain
Abstract:We reported previously that the expression of Wnt-related genes is lower in osteoporotic hip fractures than in osteoarthritis. We aimed to confirm those results by analyzing β-catenin levels and explored potential genetic and epigenetic mechanisms involved.
Keywords:AzadC  5-aza-2-deoxy-azacytidine  CACYBP  calcyclin binding protein  CAMK2G  calcium/calmodulin-dependent protein kinase II gamma  CSNK1A1  casein kinase 1  alpha 1  CSNK1A1L  casein kinase 1  alpha 1-like  CSNK1E  casein kinase 1  epsilon  Ct  threshold cycle  CTBP1  C-terminal binding protein 1  FDR  false discovery rate  FOSL1  FOS-like antigen 1  FRZB  frizzled-related protein  FZD10  frizzled homolog 10  GSK3B  glycogen synthase kinase 3 beta  GWAS  genome-wide association study  HWE  Hardy&ndash  Weinberg equilibrium  LRP5  lipoprotein receptor related protein 5  PLCB3  phospholipase C  beta 3 (phosphatidylinositol-specific)  PPP2R1A  protein phosphatase 2 (formerly 2A)  regulatory subunit A  alpha isoform  RHOA  ras homolog gene family  member A  SFRP1  secreted frizzled-related protein 1  SFRP4  secreted frizzled-related protein 4  TBL1X  transducin (beta)-like 1X-linked  TBP  TATA box binding protein  WNT10A  wingless-type MMTV integration site family  member 10A  WNT16  wingless-type MMTV integration site family  member 16  WNT4  wingless-type MMTV integration site family  member 4  WNT8A  wingless-type MMTV integration site family  member 8A
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