Characterization of Moroccan Isolates of Verticillium dahliaeKleb Using RAPD Markers |
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| Authors: | M Cherrab M N Serrhini P M Charest |
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| Institution: | Authors addresses: Department de biologie, Facultédes Sciences, UniversitéChouaib Doukkali, El Jadida, Morocco;;Department de Phytopathologie, Ecole Nationale d'Agriculture, Meknes, Morocco;;Department de phytologie, Pavillon C-E. Marchand, UniversitéLaval, Québec, Canada |
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| Abstract: | Isolates of Verticillium dahliae were sampled from different olive tree orchards in Morocco. These olive trees were located in different commercial culture locations in southern, central and northern Morocco. The isolates were characterized using genetic markers obtained after their DNA PCR amplification with random amplified polymorphic DNA (RAPD) primers. Among the 40 primers tested, 10 generated a total of 66 polymorphic fragments. Among the 38 isolates of V. dahliae tested, RAPD markers were successful in the characterization of groups based on their geographic origin. With the exception of one specific isolate, no correlation could be established among the isolates, based on the morphological appearance of the colony in culture. |
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| Keywords: | Verticillium dahliae olive tree (Olea europea var sativa) Morocco random amplified polymorphic DNA (RAPD) |
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